What is the format of the wav file, or where did you generate it from?
There are lots of options for the format of a wav file. Not all are currently supported. See more details here:
https://pynq.readthedocs.io/en/v2.0/pynq_package/pynq.lib/pynq.lib.audio.html#pynq-lib-audio
Cathal
Do you have a wav file format…can you send it
You can record one from the board and use the same one to playback.
Cathal