Hi, @cathalmccabe Sorry for the delay.
Basically the user manual for pynq z2 on TUL site has an outdated pdf that has wrong pinouts.
You can refer to their own updated pdf on this foum thread:
Is it possible to ask TUL to fix their pdf link on their website? @Colorado_Rob has even provided us the official updated pinout for pynq z2.
Appreciate your support.