Hi,
I have python script running OK on PYNQ Z2 with image V2.6, but have encountered error when the board was upgraded to image version 3.0.1. The error is "ModuleNotFoundError: No module named ‘pynq’, when trying from pynq import Overlay in the script. Same error happened to image V2.7. It seems being a compatibility issue.
Please advise how to resolve this problem. Thanks.
Need more info on the upgrade you had done, reimage the SD card?
While can Jupyter notebook terminal python import pynq return normal?
These two elements should have a good start to us what is going on.
Enjoy
@Luke2020 Are you sure you using the right python interpreter on the 3.0.1 version?
As @briansune more details would be very useful
Hi @Luke2020,
Are you running the script in the terminal? Since v2.7 we have been using a python virtual environment which you might need to activate. Can you try sourcing the following in your terminal before running your script?
source /etc/profile.d/pynq_venv.sh
If that doesn’t solve it we probably need a bit more info, as @briansune and @giop98 said 
All the best,
Shane