Hi @RoyMeijer,
Welcome to the PYNQ community.
The AXI Direct Memory Access IP do not have TUSER side-band channel on the AXI4-Stream interfaces.
You can include a FIFO in the datapath and set the TUSER using an AXI GPIO. Something like this
You then can use the AXI GPIO object to set the value of TUSER from your Python code.
Mario
